Micropropagation and Growth of in Vitro Pineapple (ananas Comosus L. Merr) in Iran
نویسنده
چکیده
Although, several pineapple micropropagation protocols have already been published, significant improvement could be achieved, if the stages of in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Tissue culture experiments were therefore conducted to develop rapid multiplication procedures for Ananas comosus L. (Merr). Terminal buds from suckers were treated with 0.025% (w/v) mercuric chloride for 2 minutes and placed in different media. Explants were transferred to MS medium supplemented with NAA (2 mgl-1) and BA ( 0, 1, 2, 3, 4, 5, 6, 7 mgl-1) and kept for 2-4 months under 16/8 h photoperiod (40 μmol m-2 s-1) and 25 ± 2oC. Results showed that higher multiplication rates for Ananas comosus L. were obtained with BA concentrations of 5 mgl-1 at 3 months. The in vitro proliferated shoots produced roots with maximum frequency (84%) on MS medium without growth regulator at 6 weeks intervals. Using the protocol described in this work, it is possible to obtain 1 million rooted plantlets after 12 months from a single bud, with a 45 day subculture interval.
منابع مشابه
An Efficient and Cost Effective Protocol for In Vitro Propagation of Pineapple
An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...
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